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FY2021-2025 Grant-in-Aid for Transformative Research Areas (A)
“Bottom-up creation of cell-free molecular systems: surpassing nature”

Research Achievements

Papers

  1. Effective design of PEGylated polyion complex (PIC) nanoparticles for enhancing PIC internalisation in cells utilising block copolymer combinations with mismatched ionic chain lengths
    Fadlina Aulia, Hiroaki Matsuba, Shoya Adachi, Takumi Yamada, Ikuhiko Nakase, Teruki Nii, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura
    J. Mater. Chem. B, 2024, in press.

  2. Preparation of cellular membrane-mimicking glycopolymer interfaces by the solvent-assisted method on QCM-D sensor chips and their molecular recognition
    Masanori Nagao, Tsukuru Masuda, Madoka Takai and Yoshiko Miura
    J. Mater. Chem. B, 2024, in press.

  3. Lipid vesicle-based molecular robots
    Zugui Peng, Shoji Iwabuchi, Kayano Izumi, Sotaro Takiguchi, Misa Yamaji, Shoko Fujita, Harune Suzuki, Fumika Kambara, Genki Fukasawa, Aileen Cooney, Lorenzo Di Michele, Yuval Elani, Tomoaki Matsuura and Ryuji Kawano
    Lab Chip, 2024, in press.

  4. Evolution of hierarchy and irreversibility in theoretical cell differentiation model
    Yoshiyuki T Nakamura, Yusuke Himeoka, Nen Saito, Chikara Furusawa
    PNAS Nexus, 2024, 3(1), pgad454

  5. Real-time monitoring of the amyloid β1–42 monomer-to-oligomer channel transition using a lipid bilayer system
    Yuri Numaguchi, Kaori Tsukakoshi, Nanami Takeuchi, Yuki Suzuki, Kazunori Ikebukuro, Ryuji Kawano
    PNAS Nexus, 2024, 3(1), pgad437

  6. Multimolecular Competition Effect as a Modulator of Protein Localization and Biochemical Networks in Cell-Size Space
    Saki Nishikawa, Gaku Sato, Sakura Takada, Shunshi Kohyama, Gen Honda, Miho Yanagisawa, Yutaka Hori, Nobuhide Doi, Natsuhiko Yoshinaga, Kei Fujiwara
    Adv. Sci., 2023, e2308030.

  7. Multistep conformational changes leading to the gate opening of light-driven sodium pump rhodopsin
    Yukino Sato, Tsubasa Hashimoto, Koji Kat, Akiko Okamura, Kaito Hasegawa, Tsukasa Shinone, Yoshikazu Tanaka, Yoshiki Tanaka, Tomoya Tsukazaki, Takashi Tsukamoto, Makoto Demura, Min Yao, Takashi Kikukawa
    J. Biol. Chem., in press.

  8. Correlation between Self-Folding Behavior of Amphiphilic Polymers and Their Molecular Flexibility
    Masanori Nagao and Yoshiko Miura
    ACS Macro Lett., 2023, 12, 6, 733-737.

  9. Engineering metabolic cycle-inspired hydrogels with enzyme-fueled programmable transient volume changes
    Young Kyoung Hong, Masahiko Nakamoto and Michiya Matsusaki
    J. Mater. Chem. B, 2023, 11, 8136-8141.

  10. Elastic Polymer Coated Nanoparticles with Fast Clearance for 19F MR Imaging
    Yuki Konishi, Masafumi Minoshima, Kohei Fujihara, Takayuki Uchihashi, Kazuya Kikuchi
    Angew. Chem. Int. Ed., 2023, 1433-7851.

  11. Iron azaphthalocyanine electrocatalysts for enhancing oxygen reduction reactions under neutral conditions and power density in microbial fuel cells
    Edwin Osebe Nyangau, Hiroya Abe, Yuta Nakayasu, Masaki Umetsu, Masaru Watanabe, Chika Tada
    Bioresource Technology Reports, 2023, 101565.

  12. Structural requirements of a glycolipid MPIase for membrane protein integration
    Kohki Fujikawa, Youjung Han, Tsukiho Osawa, Shoko Mori, Kaoru Nomura, Maki Muramoto, Ken-ichi Nishiyama, Keiko Shimamoto
    Chem. Eur. J., 2023, 29(30), e202300437.

  13. Chitin- and Streptavidin-Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery
    Shunsuke Kato, Koki Takeuchi, Motonao Iwaki, Kentaro Miyazaki, Kohsuke Honda, Takashi Hayashi
    Angew. Chem. Int. Ed., 2023, e202303764.

  14. Nanofluidic Aptamer Nanoarray to Enable Stochastic Capture of Single Proteins at Normal Concentrations
    Jinbin Yang, Hiroki Kamai, Yong Wang, Yan Xu
    Small, 2023, 2301013.

  15. A simple chemical method to nondestructively regenerate functional nanochannels for single-molecule studies
    Jinbin Yang, Hiroki Kamai and Yan Xu
    Sensors and Actuators B: Chemical, 2023, 134106.

  16. Mussel-inspired interfacial ultrathin films for cellular adhesion on the wrinkled surfaces of hydrophobic fluids
    Hiroya Abe, Tomoya Ina, Hirokazu Kaji and Matsuhiko Nishizawa
    Polymer Journal, 2023, 1-6.

  17. Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules Using a Biological Nanopore
    Asuka Tada, Nanami Takeuchi, Kan Shoji, and Ryuji Kawano
    Anal. Chem., 2023, ASAP.

  18. Investigation of Compatibility between DNA Replication, Transcription, and Translation for in Vitro Central Dogma
    Kaito Seo and Norikazu Ichihashi
    ACS Synth. Biol., 2023, 12, 6, 1813-1822.

  19. Self-assembled GA-Repeated Peptides as a Biomolecular Scaffold for Biosensing with MoS2 Electrochemical Transistors
    Hironaga Noguchi, Yoshiki Nakamura, Sayaka Tezuka, Takakazu Seki, Kazuki Yatsu, Takuma Narimatsu, Yasuaki Nakata, and Yuhei Hayamizu
    ACS Appl. Mater. Interfaces, 2023, 15, 11, 14058–14066.

  20. Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates
    Biplab K C, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura
    Chem. Sci., 2023, 14, 6608-6620.

  21. Spatiotemporal Control of Ice Crystallization in Supercooled Water via an Ultrashort Laser Impulse
    Hozumi Takahashi, Tatsuya Kono, Kosuke Sawada, Satoru Kumano, Yuka Tsuri, Mihoko Maruyama, Masashi Yoshimura, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura, Seiichiro Nakabayashi, Yusuke Mori, Yoichiroh Hosokawa, and Hiroshi Y. Yoshikawa
    J. Phys. Chem. Lett., 2023, 14, 19, 4394–4402.

  22. Flexible Glass-Based Hybrid Nanofluidic Device to Enable the Active Regulation of Single-Molecule Flows
    Hiroto Kawagishi, Shun-ichi Funano, Yo Tanaka, and Yan Xu
    Nano Lett., 2023, 23, 6, 2210-2218.

  23. Phenylazothiazoles as Visible-Light Photoswitches
    Runze Lin, P. K. Hashim, Saugata Sahu, Ammathnadu S. Amrutha, Nusaiba Madappuram Cheruthu, Shakkeeb Thazhathethil, Kiyonori Takahashi, Takayoshi Nakamura, Takashi Kikukawa, and Nobuyuki Tamaoki
    J. Am. Chem. Soc., 2023, 145, 16, 9072-9080.

  24. Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies
    Ryotaro Utsumi, Yuki Murata, Sayoko Ito-Harashima, Misaki Akai, Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka
    Plos one, 2023, in press.

  25. In Vitro Transcription/Translation-Coupled DNA Replication through Partial Regeneration of 20 Aminoacyl-tRNA Synthetases
    Katsumi Hagino, Norikazu Ichihashi
    ACS Synth. Biol., 2023, 12, 4, 1252-1263.

  26. Increased Enzyme Loading in PICsomes via Controlling Membrane Permeability Improves Enzyme Prodrug Cancer Therapy Outcome
    Akinori Goto, Yasutaka Anraku, Shigeto Fukushima, Akihiro Kishimura
    Polymers, 2023, 15, 1368.

  27. Characterization of retinal chromophore and protonated Schiff base in Thermoplasmatales archaeon heliorhodopsin using solid-state NMR spectroscopy
    Shibuki Suzuki, Sari Kumagai, Toshio Nagashima, Toshio Yamazaki, Takashi Okitsu, Akimori Wada, Akira Naito, Kota Katayama, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
    Biophys. Chem., 2023, 296, 106991.

  28. Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Barrel Protein Scaffold
    Shunsuke Kato, Akira Onoda, Ulrich Schwaneberg, Takashi Hayashi
    J. Am. Chem. Soc., 2023, 145, 15, 8285-8290.

  29. Development of a Versatile Protein Labeling Tool for Live-Cell Imaging Using Fluorescent ß-Lactamase Inhibitors
    Masafumi Minoshima, Taro Umeno, Kohei Kadooka, Margaux Roux, Namiko Yamada, Kazuya Kikuchi
    Angew. Chem. Int. Ed., 2023, in press.

  30. Toward nanofluidics-based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds
    Nattapong Chantipmanee, Yan Xu
    VIEW, 2023, 4, 1, 20220036
    本誌表紙に採択されました

  31. Cell-free expression of de novo designed peptides that form β-barrel nanopores
    Shoko Fujita, Izuru Kawamura, Ryuji Kawano
    ACS Nano, 2023, 17, 4, 3358.

  32. Liposome Deformation Induced by Membrane-Binding Peptides
    Kayano Izumi, Chihiro Saito, Ryuji Kawano
    Micromachines, 2023, 14, 373.

  33. Facile preparation of hexagonal nanosheets via polyion complex formation from α-helical polypeptides and polyphosphate-based molecules
    Asmariah Ahmad, Tomoki Maruyama, Teruki Nii, Takeshi Mori, Yoshiki Katayama. Akihiro Kishimura
    Chem comm., 2023, 59, 1657–1660.

  34. Designable peptides on graphene field-effect transistors for selective detection of odor molecules
    Chishu Homma, Mirano Tsukiiwa, Hironaga Noguchi, Masayoshi Tanaka, Mina Okochi, Hideyuki Tomizawa, Yoshiaki Sugizaki, Atsunobu Isobayashi, Yuhei Hayamizu
    Biosens. Bioelectron., 2023, 224, 115047.

  35. Emergence of growth and dormancy from a kinetic model of the Escherichia coli central carbon metabolism
    Yusuke Himeoka and Namiko Mitarai
    Phys. Rev. Research, 2022, 4, 043223.

  36. Surfactant-Assisted Purification of Hydrophobic DNA Nanostructures
    Shoji Iwabuchi, Shin-ichiro M. Nomura, Yusuke Sato
    ChemBioChem, 2022, e202200.

  37. ePURE_JSBML: A tool for constructing a deterministic model of a reconstituted Escherichia coli protein translation system with a user-specified nucleic acid sequence
    Yoshihiro Shimizu, Naoki Tanimura, Tomoaki Matsuura
    Adv. Biology, 2022, 2200177.

  38. Plausible pathway for a host-parasite molecular replication network to increase its complexity through Darwinian evolution
    Rikuto Kamiura,Ryo Mizuuchi,Norikazu Ichihashi
    PLoS Comput. Biol.,2022, 1010709.

  39. Fabrication of a polymeric inhibitor of proximal metabolic enzymes in hypoxia for synergistic Inhibition of cancer cell proliferation, survival, and migration
    Yuki Koba, Masahiko Nakamoto, Michiya Matsusaki
    ACS Appl. Mater. Interfaces, 2022, 14, 46, 51790−51797.

  40. A peptide nanocage constructed by self-assembly of oligoproline conjugates
    Shogo Matsubara, Yui Okamoto, Masaru Yoshikawa, Shinya Tsukiji, Masahiro Higuchi
    Bioconj. Chem., 2022, 33, 10, 1785-1788.

  41. A methodology for creating thermostabilized mutants of G-protein coupled receptors by combining statistical thermodynamics and evolutionary molecular engineering
    Kanna Sugaya, Satoshi Yasuda, Shingo Sato, Chen Sisi, Taisei Yamamoto, Daisuke Umeno, Tomoaki Matsuura, Tomohiko Hayashi, Satoshi Ogasawara, Masahiro Kinoshita, Takeshi Murata
    Protein Sci., 2022, 31, 9, e4404.

  42. Three-dimensional structure of the antimicrobial peptide cecropin P1 in dodecylphosphocholine micelles and the role of the C-terminal residues
    Hao Gu, Takasumi Kato, Hiroyuki Kumeta, Yasuhiro Kumaki, Takashi Tsukamoto, Takashi Kikukawa, Makoto Demura, Hiroaki Ishida, Hans J. Vogel, Tomoyasu Aizawa
    ACS Omega, 2022, 7, 36, 31924−31934.

  43. Protein-structure-dependent spectral shifts of near-infrared photoluminescence from locally functionalized single-walled carbon nanotubes based on avidin–biotin interactions
    Yoshiaki Niidome, Rie Wakabayashi, Masahiro Goto, Tsuyohiko Fujigaya, Tomohiro Shiraki
    Nanoscale, 2022, 14, 13090–13097.

  44. Molecular crowding elicits the acceleration of enzymatic crosslinking of macromolecular substrates
    Ryo Sato, Kosuke Minamihata, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya
    Org. Biomol. Chem., 2022, 21, 306–314.

  45. Effects of pulse duration on laser-induced crystallization of urea from 300 to 1200 fs: impact of cavitation bubbles on crystal nucleation
    Yuka Tsuri, Mihoko Maruyama, Katsuo Tsukamoto, Hiroaki Adachi, Kazufumi Takano, Shigeyoshi Usami, Masayuki Imanishi, Masashi Yoshimura, Hiroshi Y. Yoshikawa, Yusuke Mori
    Appl. Phys. A, 2022, 128, 803.

  46. Synthesis of well-defined cyclic glycopolymers and the relationship between their physical properties and their interaction with lectins
    Masanori Nagao, Yu Hoshino, Yoshiko Miura
    Polym. Chem., 2022, 13, 5453–5457.

  47. Mechanical guidance of self-condensation patterns of differentiating progeny
    Takahisa Matsuzaki, Yuko Shimokawa, Hiroyuki Koike, Masaki Kimura, Yuma Kawano, Nao Okuma, Ryuzo Kawamura, Yosuke Yoneyama, Yasuro Furuichi, Fumihiko Hakuno, Shin-Ichiro Takahashi, Seiichiro Nakabayashi, Satoshi Okamoto, Hiromitsu Nakauchi, Hideki Taniguchi, Takanori Takebe, Hiroshi Y. Yoshikawa
    iScience, 2022, 25, 10, 105109.

  48. Practice of responsible research and innovation in the formulation and revision of ethical principles of molecular robotics in Japan
    Ken Komiya, Ryuma Shineha, Naoto Kawahara
    SN Appl. Sci., 2022, 4, 305.

  49. Experimental evidence for the correlation between RNA structural fluctuations and the frequency of beneficial mutations
    Yutaro Maeda, Ryo Mizuuchi, Shuji Shigenobu, Atsushi Shibai, Hazuki Kotani, Chikara Furusawa, Norikazu Ichihashi
    RNA, 2022, 28, 12, 1659-1667.

  50. Controlling the periodicity of a reaction–diffusion wave in artificial cells by a two-way energy supplier
    Sakura Takada, Natsuhiko Yoshinaga, Nobuhide Doi, Kei Fujiwara
    ACS Nano, 2022, 16, 10, 16853−16861.

  51. Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant
    Yuhei Doi, Jo Watanabe, Ryota Nii, Takashi Tsukamoto, Makoto Demura, Yuki Sudo, Takashi Kikukawa
    Sci. Rep., 2022, 12, 16422.

  52. A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation
    Sachio Suzuki, Akinobu Nakamura, Yuka Hatano, Masaru Yoshikawa, Tatsuyuki Yoshii, Shunsuke Sawada, Kyoko Atsuta-Tsunoda, Kazuhiro Aoki, Shinya Tsukiji
    Cell Chem. Biol., 2022, 29, 9, 1446-1464.e10.

  53. Reisomerization of retinal represents a molecular switch mediating Na+ uptake and release by a bacterial sodium-pumping rhodopsin
    Tomotsumi Fujisawa, Kouta Kinoue, Ryouhei Seike, Takashi Kikukawa, Masashi Unno
    J. Biol. Chem., 2022, 298, 9, 102366.

  54. Biomacromolecule-fueled transient volume phase transition of a hydrogel
    Masahiko Nakamoto, Shiro Kitano, Michiya Matsusaki
    Angew. Chem. Int. Ed., 2022, 61, 33,e202205125.

  55. Role of a bacterial glycolipid in Sec-independent membrane protein insertion
    Kaoru Nomura, Shoko Mori, Kohki Fujikawa, Tsukiho Osawa, Shugo Tsuda, Kumiko Yoshizawa-Kumagaye, Shun Masuda, Hideki Nishio, Taku Yoshiya, Takao Yoda, Masafumi Shionyu, Tsuyoshi Shirai, Ken-ichi Nishiyama, Keiko Shimamoto
    Sci. Rep., 2022, 12, 12231.

  56. Transfer RNA synthesis-coupled translation and DNA replication in a reconstituted transcription/translation system
    Ryota Miyachi, Yoshihiro Shimizu, Norikazu Ichihashi
    ACS Synth. Biol., 2022, 11 , 8,2791-2799.

  57. One-step preparation of cell-free ATP regeneration module based on non-oxidative glycolysis using thermophilic enzymes
    Gladwin Suryatin Alim, Kenji Okano, Kohsuke Honda
    ChemBioChem, 2022, 23, 16,e202200210.

  58. Pattern recognition of microRNA expression in body fluids using nanopore decoding at subfemtomolar concentrations
    Nanami Takeuchi, Moe Hiratani, Ryuji Kawano
    JACS Au, 2022, 2, 9, 1829–1838.
    プレスリリース:ナノポアを用いた血中がんマーカーのパターン識別に成功!―DNAコンピューティング技術による並列計算を利用―, 東京農工大学
    メディア報道:日本経済新聞, EurekAlert!, Bioengineer.org, SCIENMAG, TECH+, GEN, ecancer, Jano.es, eBiotrade, List23, tetracyclined7k, AZoLifeSciences

  59. A photoactivatable self-localizing ligand with improved photosensitivity for chemo-optogenetic control of protein localization in living cells
    Tatsuyuki Yoshii, Choji Oki, Shinya Tsukiji
    Bioorg. Med. Chem. Lett., 2022, 72, 128865.

  60. A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells
    Hideyuki Nakanishi, Tatsuyuki Yoshii, Shinya Tsukiji, Hirohide Saito
    STAR Protoc., 2022, 101451.

  61. One-step preparation of cell-free ATP regeneration module based on non-oxidative glycolysis using thermophilic enzymes
    Gladwin Suryatin Alim, Kenji Okano, Kohsuke Honda
    ChemBioChem,, 2022, e202200210.

  62. Fluorescent hydrogel based on self-assembling acridonylalanine-phenylalanine
    Yuzuha Araki, Hiroki Shirakata, Tetsuya Nakagawa, Takashi Ubukata, Yasushi Yokoyama, Izuru Kawamurabr
    Chem. Lett., 2022, 51, 7, 687–689.

  63. Functionalized silicone elastomer via alkaline solution to coat phosphorylcholine-based copolymer containing organosilane to improve hemocompatibility for medical devices
    Fang-Yu Chou, Shintaro Hara, Kazuto Uchida, Youichi Matsuo, Tsukuru Masuda, Ryo Yokoi, Toshiya Ono, Masaki Anraku, Takashi Isoyama, Madoka Takai
    Front. Mater., 2022, 9, 877755.

  64. Construction of a reduction-responsive DNA microsphere using a reduction-cleavable spacer based on a nitrobenzene scaffold
    Sayuri L. Higashi, Ayaka Isogami, Junko Takahashi, Aya Shibata, Koichiro M. Hirosawa, Kenichi G.N. Suzuki, Shunsuke Sawada, Shinya Tsukiji, Kazunori Matsuura, Masato Ikeda
    Chem. Eur. J., 2022, 17, 10, e202200142.

  65. Photoreaction pathways of bacteriorhodopsin and its D96N mutant as revealed by in-situ photoirradiation solid-state NMR
    Arisu Shigeta, Yuto Otani, Ryota Miyasa, Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Akira Naito
    Membranes, 2022, 12, 3, 279.

  66. Silver-loaded carboxymethyl cellulose nonwoven sheet with controlled counterions for infected wound healing
    Seiichi Ohta, Kento Mitsuhashi, Arvind K. Singh Chandel, Pan Qi, Noriko Nakamura, Akiko Nakamichi, Hiromi Yoshida, Gento Yamaguchi, Yuichi Hara, Ryo Sasaki, Masaya Fuke, Madoka Takai, Taichi Ito
    Carbohydr. Polym., 2022, 286, 119289.

  67. Cell-free synthesis of human endothelin receptors and its application to ribosome display
    Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe, and Tomoaki Matsuura
    Anal. Chem., 2022, 94, 9, 3831–3839.

  68. Functional visualization of NK cell-mediated killing of metastatic single tumor cells
    Hiroshi Ichise, Shoko Tsukamoto, Tsuyoshi Hirashima, Yoshinobu Konishi, Choji Oki, Shinya Tsukiji, Satoshi Iwano, Atsushi Miyawaki, Kenta Sumiyama, Kenta Terai, Michiyuki Matsuda
    eLife, 2022, 11, e76269.

  69. Review “Recent progress and perspectives in applications of 2-methacryloyloxyethyl phosphorylcholine polymers in biodevices at small scales
    Sasikarn Seetasang, Yan Xu
    J. Mater. Chem. B,2022, 10, 14, 2323–2337.

  70. Review “Condensate Formation by Metabolic Enzymes in Saccharomyces cerevisiae
    Natsuko Miura
    Microorganisms, 2022, 10, 2, 232.

  71. A biomimetic anti-biofouling coating in nanofluidic channels
    Sumire Fukuda, Yan Xu
    J. Mater. Chem. B, 2022, 10, 14, 2481–2489.
    Journal of Materials Chemistry B HOT Papersに選出された

  72. Chimeric mutants of staphylococcal hemolysin, which actas both one-component and two-component hemolysin, created by grafting the stem domain
    Nouran Ghanem, Natsuki Kanagami, Takashi Matsui, Kein Takeda, Jun Kaneko, Yasuyuki Shiraishi, Christian A. Choe, Tomomi Uchikubo-Kamo, Mikako Shirouzu, Tsubasa Hashimoto, Tomohisa Ogawa, Tomoaki Matsuura, Po-Ssu Huang, Takeshi Yokoyama, Yoshikazu Tanaka
    FEBS J., 2022, 289, 12, 3505–3520.

  73. Review “Design of biointerfaces composed of soft materials using controlled radical polymerization
    Tsukuru Masuda, Madoka Takai
    J. Mater. Chem. B, 2022, 10, 10, 1473–1485.

  74. Photoswitching of 5-phenylazopyrimidines in crystalline powders and thin films
    Eliška Procházková, Juraj Filo, Lucie Mužíková Čechová, Martin Dračínský, Ivana Císařová, Zlatko Janeba, Izuru Kawamura, Akira Naito, Peter Nádaždy, Peter Šiffalovič, Marek Cigáň
    Dyes Pigm., 2022, 199, 110066.

  75. Cell adhesion and migration on thickness gradient bilayer polymer brush surfaces: effects of properties of polymeric materials of the underlayer
    Zahra Afzali, Taishi Matsushita, Akinori Kogure, Tsukuru Masuda, Tomoyuki Azuma, Keiichiro Kushiro, Toshihiro Kasama, Ryo Miyake, Madoka Takai
    ACS Appl. Mater. Interfaces, 2022, 14, 2, 2605–2617.

  76. Review “Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces
    Katsutoshi Hori, Shogo Yoshimoto, Tomoko Yoshino, Tamotsu Zako, Gen Hirao, Satoshi Fujita, Chikashi Nakamura, Ayana Yamagishi, Noriho Kamiya
    J. Biosci. Bioeng., 2022, 133, 3, 195–207.

  77. Two-dimensional imaging of a model pharmaceutical dosage tablet using a scanning vibrational circular dichroism system
    Hisako Sato, Sumio Aisawa, Honoka Ida, Masaru Shimizu, Keisuke Watanabe, Jun Koshobu, Jun Yoshida, Izuru Kawamura
    Chem. Lett., 2022, 51, 2, 205-207.

  78. Nanofluidics for sub-single cellular studies: Nascent progress, critical technologies, and future perspectives
    Jinbin Yang, Yan Xu
    Chin. Chem. Lett., 2022, 33, 6, 2799.

  79. De novo design of a nanopore for single molecule detection that incorporates a β-hairpin peptide
    Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada, Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano
    Nature Nanotechnol., 2022, 17, 67.

  80. Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system
    Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori, Tomoaki Matsuura
    J. Biosci. Bioeng., 2022, 133, 2, 181.

  81. A Dual Promoter System to Monitor IFN-γ Signaling in vivo at Single-cell Resolution
    Taisei Tanaka, Yoshinobu Konishi, Hiroshi Ichise, Shinya Tsukiji, Michiyuki Matsuda, Kenta Terai
    Cell Struct. Funct., 2022, 46, 2, 103

  82. pH-responsive water-soluble polymer carriers for cell-selective metabolic sialylation labeling
    Tingbi Zhao, Tsukuru Masuda, Madoka Takai
    Anal. Chem., 2021, 93, 46, 15420.

  83. Viewpoint “ Modularly built synthetic membraneless organelles enabling targeted protein sequestration and release
    Shuhao Lin, Daiki Hirayama, Gembu Maryu, Kimiya Matsuda, Naoya Hino, Eriko Deguchi, Kazuhiro Masaru Yoshikawa, Shinya Tsukiji
    Biochemistry, 2021, 60, 44, 3273.

  84. Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration
    Shuhao Lin, Daiki Hirayama, Gembu Maryu, Kimiya Matsuda, Naoya Hino, Eriko Deguchi, Kazuhiro Aoki, Ryo Iwamoto, Kenta Terai, Michiyuki Matsuda
    Life Sci. Alliance, 2021, 5, e202101206.

  85. Fabrication of nanoscale gas–liquid interfaces in hydrophilic/hydrophobic nanopatterned nanofluidic channels
    Hiroto Kawagishi, Shuichi Kawamata, Yan Xu
    Nano Lett., 2021, 21, 24, 10555.
    掲載号(2021年21巻24号)の表紙に採択
    プレスリリース:ナノスケールで物質の濃縮効果を発見―ナノ流体デバイスによる超微小気液界面の作製と効果―, 大阪府立大学・JST
    メディア報道:日刊工業新聞, EurekAlert!, Asia Research News, Phys.org, Nanowerk, chemeurope.com, SCIENMAG, True Viral News, Mirage News

Commercialized, patents

  1. 2022.07.14
    A01 Tsukiji group
    Reagent for inducing membrane localization of proteins「SLIPT-PM」on sale(Funakoshi)
    https://www.funakoshi.co.jp/contents/70609
    https://www.funakoshi.co.jp/contents/70604

Press Releases & Media Coverage

  1. Kasano group:JACS Aurelated

    Press Releases
    Successful Pattern Discrimination of Cancer Markers in Blood Using Nanopores! -Using Parallel Computing with DNA Computing Technology―, TUAT
    https://www.tuat.ac.jp/outline/disclosure/pressrelease/2022/20220627_01.html

    Media coverage
    Tokyo University of Agriculture and Technology succeeded in identifying patterns of cancer markers in blood using nanopores., Nikkei Net, 27 Jun 2022, 
    https://www.nikkei.com/article/DGXZRSP635247_X20C22A6000000/

    Detection of cancer biomarkers from blood samples using nanopore-based DNA computing technology, EurekAlert!, 27 Jun 2022
    https://www.eurekalert.org/news-releases/957090

    Detection of cancer biomarkers from blood samples using nanopore-based DNA computing technology, Bioengineer.org, 27 Jun 2022
    https://bioengineer.org/detection-of-cancer-biomarkers-from-blood-samples-using-nanopore-based-dna-computing-technology/

    Detection of cancer biomarkers from blood samples using nanopore-based DNA computing technology, ScienMag, 27 Jun 2022
    https://scienmag.com/detection-of-cancer-biomarkers-from-blood-samples-using-nanopore-based-dna-computing-technology/

    TUAT succeeded in identifying cholangiocarcinoma by DNA computing and nanopore measurement., TECH+, 28 Jun 2022
    https://news.mynavi.jp/techplus/article/20220628-2382598/

    Nanopore Technology Detects Cancer Biomarkers from Patients’ Blood, GEN, 28 Jun 2022
    https://www.genengnews.com/topics/translational-medicine/biomarkers/nanopore-technology-detects-cancer-biomarkers-from-patients-blood/

    Detection of cancer biomarkers from blood samples using nanopore-based DNA computing technology, ecancer, 28 Jun 2022
    https://ecancer.org/en/news/22000-detection-of-cancer-biomarkers-from-blood-samples-using-nanopore-based-dna-computing-technology

    Nuevo método para detectar biomarcadores de cáncer con muestras de sangre, Jano, 29 Jun 2022 
    https://www.jano.es/noticia-nuevo-metodo-detectar-biomarcadores-cancer-33440

    利用纳米孔DNA技术从血液样本中检测癌症生物标志物, ebiotrade, 29 Jun 2022
    https://www.ebiotrade.com/newsf/2022-6/20220628002541949.htm

    Cancer Biomarkers are detected from patients’ blood using nanopore technology, list23, 29 Jun 2022
    https://list23.com/936881-cancer-biomarkers-are-detected-from-patients-blood-using-nanopore-technology/

    Nanopore Technology Detects Cancer Biomarkers from Patients’ Blood, tetracyclined7k, 29 Jun 2022
    https://tetracyclined7k.com/nanopore-technology-detects-cancer-biomarkers-from-patients-blood/

    Researchers use nanopore-based DNA computing technology to detect cancer, AZO Life Sciences, 1 Jul 2022
    https://www.azolifesciences.com/news/20220701/Researchers-use-nanopore-based-DNA-computing-technology-to-detect-cancer.aspx

  2. Xu group:Nano Lettrelated

    Press Releases
    “Discovery of Material Concentration Effects at the Nanoscale – Fabrication and Effects of Ultra-Micro Gas-Liquid Interfaces Using Nanofluidic Devices”、Joint press release from Osaka Prefecture University and JST(Oct/14/2021)

    ▼Osaka Prefecture University website
    https://www.osakafu-u.ac.jp/press-release/pr20211014_3/

    ▼JST website
    https://www.jst.go.jp/pr/announce/20211014-3/index.html

    ▼Osaka Public University
    https://www.upc-osaka.ac.jp/new-univ/research/press_release/

    ▼Osaka Prefecture University Twitter
    https://twitter.com/OsakaPrefUniv/status/1448544485890166788

    ▼Osaka Prefecture University Facebook
    https://www.facebook.com/OsakaPrefectureUniv/posts/267661358698236

    ▼YouTube
    https://www.youtube.com/watch?v=ggQIXIDelgQ

    Media coverage
    Osaka Prefectural Univ. succeeds in fabricating nanoscale ultra-small gas-liquid interfaces with high precision and uniformity,Nikkan Kogyo Shimbun , Electric version、(Oct/15/2021)
    https://www.nikkan.co.jp/spaces/view/0061628

    First controllable nanoscale gas-liquid interface fabricated
    EurekAlert!
    https://www.eurekalert.org/news-releases/931973

    Asia Research News
    https://www.asiaresearchnews.com/content/first-controllable-nanoscale-gas-liquid-interface-fabricated

    Phys.orgPhys.org
    https://phys.org/news/2021-10-nanoscale-gas-liquid-interface-fabricated.html

    Nanowerk
    https://www.nanowerk.com/nanotechnology-news2/newsid=58952.php

    Chem Europe
    https://www.chemeurope.com/en/news/1173219/first-controllable-nanoscale-gas-liquid-interface-fabricated.html

    ScienMag
    https://scienmag.com/first-controllable-nanoscale-gas-liquid-interface-fabricated/

    True Viral News
    https://trueviralnews.com/52861-first-controllable-nanoscale-gas-liquid-interface-fabricated.html

    Mirage News
    https://www.miragenews.com/first-controllable-nanoscale-gas-liquid-655001/

    Nano LettersのOfficial Twitterにて紹介, (Nov/6/2021)
    https://mobile.twitter.com/NanoLetters/status/1456697932716154883

    The research results were published in Chinese Science Media Chemistry and Materials Chemistry., (Nov/22/2021)
    https://mp.weixin.qq.com/s/0aYt3dUtEerytmr3TKnm4A

    Research Results on Nanofluidic Devices for Fabrication of Nanoscale Ultramicro Gas-Liquid Interfaces Graced the Cover of Nano Letters (Volume 21, Issue 24, 2021), (Dec/22/2021)
    https://pubs.acs.org/pb-assets/images/_journalCovers/nalefd/nalefd_v021i024-3.jpg?0.4156400251492782

  3. Xu group:J Mater Chem Brelated

    “A biomimetic anti-biofouling coating in nanofluidic channels” was selected as HOT Paper of Journal of Materials Chemistry, (Jan/20/2022)
    https://pubs.rsc.org/en/content/articlelanding/2022/tb/d1tb02627e

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